The process in which relatively unspecialized cells acquire specialized structural and/or functional features of leading edge cells, cells at the front of a migrating epithelial sheet.

Any process that stops, prevents, or reduces the frequency, rate or extent of Rho protein signal transduction.

The commitment of cells to leading edge cell fate during dorsal closure. Leading edge cells are the dorsal-most cells of the migrating epidermis.

The commitment of cells to leading edge cell fate and their capacity to differentiate into leading edge cells. Leading edge cells are found at the front of a migrating epithelial sheet.

The process in which a cell becomes capable of differentiating autonomously into a leading edge cell regardless of its environment; upon determination, the cell fate cannot be reversed.

Catalysis of the reaction: ATP + ceramide = ADP + ceramide-1-phosphate.

Catalysis of the phosphorylation of a simple or complex lipid.

A contractile actin filament bundle that consists of short actin filaments with alternating polarity, cross-linked by alpha-actinin and possibly other actin bundling proteins, and with myosin present in a periodic distribution along the fiber.

Any complex of actin, myosin, and accessory proteins.

Any process that stops, prevents, or reduces the frequency, rate or extent of Rac protein signal transduction.

Any process that modulates the frequency, rate or extent of Rac protein signal transduction.

Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.

Any process that activates or increases the frequency, rate or extent of Rac protein signal transduction.

OBSOLETE. High molecular weight (100-300 kDa) proteins that associate with intermediate filaments to cross-link them into loose networks.

OBSOLETE. Proteins that associate with the ends of intermediate filaments and couple the intermediate filaments to the plasma membrane.

OBSOLETE. Proteins that associate with intermediate filaments and function in the supramolecular organization of cellular intermediate filament networks.

Catalysis of the reaction: glycine + 2 A = HCN + CO2 + 2 AH2.

OBSOLETE. Low molecular weight (10-45 kDa) proteins that associate with intermediate filaments by lateral binding of the filaments and have the effect of creating tight macrofilament aggregates.

Catalysis of the reaction: (R)-canadine + 2 NADP(+) = berberine + H(+) + 2 NADPH.